In vitro antioxidant activity of different extract of Ganoderma lucidum

Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained a great importance. Different extract (Hot water, Hydro alcoholic, Chloroform and Petroleum Ether) of Ganoderma lucidum was studied for its in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, FRAP assay and Superoxide Radical Scavenging Assay. The results were analyzed statistically by the regression method. Its antioxidant activity was estimated by IC50 value. The results showed that the Hot water and Hydro alcoholic extracts of Ganoderma lucidum showed potent antioxidant activity than the Chloroform and Petroleum Ether extracts. The antioxidant potency of different extracts has been expressed as in order of RGHW>RGHA>RGCH>RGPEt In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Ganoderma lucidum is effective against free radical mediated disease.